Skip to main content
. 2021 Jan 15;11:611122. doi: 10.3389/fmicb.2020.611122

Figure 5.

Figure 5

Exogenous Rv1717 protein inhibits biofilm formation and degrades biofilms. (A) Wild type M. smegmatis was allowed to form biofilm in Sauton’s medium containing different concentrations (0.01–5 μM) of purified Rv1717 protein in sodium phosphate buffer/buffer only/neither (untreated). On day 3, the biofilm biomass was quantified by crystal violet assay. (B) Fifth day air-liquid interface pellicle of M. smegmatis culture in Sauton’s medium in presence of 1 μM Rv1717. (C) Mycobacterium smegmatis biofilms were treated with 5 μM Rv1717 protein in sodium phosphate buffer pH 8.0 or buffer only. The biofilm biomass was quantified by crystal violet assay. Statistical significance of data wherever applicable is indicated by ***p < 0.001. Data plotted are mean ± SD of three independent experiments.