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. 2021 Jan 26;11:621581. doi: 10.3389/fmicb.2020.621581

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Copyright © 2021 Zuzolo, Guarino, Tartaglia and Sciarrillo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mycorrhization of Lotus and Festuca roots and Rhizobium nodules of Lotus roots detected by Trypan Blue staining and light microscopy. (A,B) show the mycorrhize colonization in Festuca roots. Intraradical vesicles (v) and hyphae (h) developing intracellular microsclerotia structure (m), with rounded hyphae cell, are visible in root fragments. (C,D) show Intraradical hyphae (h) depicting an advanced arbuscule colonization in Lotus roots. (E,F) display Lotus root nodules. Outer, lighter stained, cortex of nodule is well-distinguished from the symbiotic region that is heavily infiltrated by Rhizobium bacteria.