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. 2021 Jan 21;11:624529. doi: 10.3389/fphar.2020.624529

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Copyright © 2021 Deng, Yue, Wang, Zhou, Zhou, Liu, Ni, Huang, Qiu, Liu, Tan, Tang, Wang, Ma and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALI-induced cytotoxicity on HK-2 cells was associated with ferroptosis. (A) Chemical structure of ALI. (B) Cell viability of HK-2 cells after 24 h ALI treatment was detected using CCK-8 assay. (C), (D) Intracellular GSH content in HK-2 (X200). (E) Intracellular Fe²⁺ levels in HK-2 cells treated with ALI. (F), (G) The protein levels of 4-HNE were measured by Western blot. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, ^**** p < 0.0001 vs. the control group.