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. 2021 Jan 21;11:624529. doi: 10.3389/fphar.2020.624529

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Copyright © 2021 Deng, Yue, Wang, Zhou, Zhou, Liu, Ni, Huang, Qiu, Liu, Tan, Tang, Wang, Ma and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALI-induced cytotoxicity could be alleviated by iron chelator DFO. (A) Cell viability of HK-2 cells was detected using CCK-8 assay. (B) Intracellular Fe²⁺ levels in HK-2 cells treated by ALI and DFO. (C), (D) Intracellular GSH content in HK-2 (X200). (E), (F) The protein levels of 4-HNE were measured by Western blot. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, ^**** p < 0.0001 vs. the control group; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001, ^#### p < 0.0001 vs. the ALI group.