Figure 7.

vGlut2-labeled RGC axon terminals are lost in an IOP-dependent manner in 9 m D2 mice. (A) 2.5-micron-thick maximum intensity projections of vGlut2 staining from the dLGN core of 4 m D2-control (n = six mice) and D2 mice (n = four mice). (B) Neither density nor size of detected vGlut2 puncta was significantly different between D2-control and D2 mice at 4 m. (C) vGlut2 images from 9 m D2-control (n = 10 mice) and D2 mice (n = 10 mice). For D2 mice, images are shown from mice with higher vGlut2 density (middle panel) and lower vGlut2 density (right panel) to illustrate the range of labeling seen. (D) vGlut2 density is significantly reduced in 9 m D2 mice compared to D2-controls (unpaired t-test) while vGlut2 puncta size is not different. (E) vGlut2 density in 9 m D2 mice dLGN sections significantly correlated with the 3-month cumulative IOP as assessed with linear regression.