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. 2021 Jan 21;11:599869. doi: 10.3389/fendo.2020.599869

Figure 2.

Figure 2

AR ablation results in disruption to adrenocortical markers. (A) Immunostaining of 20 α-HSD revealed an enlarged X-zone in G2 Ad-ARKO females up through the cortex, compared to WT controls were the X-zone is maintained at the cortex medulla boundary. Red: 20alpha-HSD protein, Green: sytox counterstain. (B) N=5. Immunostaining of AKR1B7 revealed loss of positive cells throughout the adrenal cortex in 1st and G2 Ad-ARKO females. Red: AKR1B7 protein, Green: sytox counterstain. N=5. Analysis of corticosterone (C), androstenedione (D), testosterone (E) and progesterone (F) reveals no changes following ablation of AR in the adrenal cortex (one-way ANOVA; n=4-8, Tukey’s post-hoc analysis, error bars SEM). Serum #72 and serum #73 were pooled serum from mouse spiked in two levels with androgens. All mice collected and analyzed at d80. Scale Bars 100µm. Annotations; M, medulla; X, X-zone; ZF, zona fasciculata.