Table 1.
Immunohistochemistry performed in this study, listing antibody source and method used.
| Protein stained for | Method | Antibody dilution | Primary antibodies |
|---|---|---|---|
| HSD3B | Single fluorescence | 1:750 | HSD3B: Santa Cruz #sc30820 |
| AR | Single fluorescence | 1:500 | AR: Spring Bioscience #M4070 |
| PCNA | DAB | 1:200 | Sigma #P-8825 |
| AKR1B7 | Single fluorescence | 1:50 | Santa Cruz Biotechnology #sc-27763 |
| HSD20alpha | Single fluorescence | 1:250 | Aviva Systems Biology #OAGA00409 |
| GR | DAB | 1:200 | Cell Signaling #12041 |
| CASP3 | Bond | 1:100 | CASP3: Abcam #ab4051 |