Table 2.
LC-MS/MS limits of quantification (LOQ).
| Serum #72 Q Low | Serum #72 Q High | Serum #73 Q Low | Serum #73 Q High | LOQ | Range | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean (nM) | RSD (%) | Recovery (%) | Mean (nM) | RSD (%) | Recovery (%) | Mean (nM) | RSD (%) | Recovery (%) | Mean (nM) | RSD (%) | Recovery (%) | (nm) | (nM) | |
| Estrone 1-sulfate | 0.43 | 7 | 105 | 0.94 | 5.7 | 99 | 0.39 | 8.7 | 96 | 0.92 | 8.9 | 96 | 0.026 | LOQ-10 |
| Cortisone | 4.63 | 0.87 | 100 | 10.6 | 7.6 | 98 | 4.2 | 4.1 | 91 | 10.4 | 3.4 | 97 | 0.19 | LOQ-112 |
| Cortisol | 35.3 | 8.1 | 102 | 81 | 2.3 | 100 | 32.3 | 0.9 | 93 | 77.7 | 1.7 | 96 | 1.9 | LOQ-794 |
| Dehydroepiandrosterone sulfate | 720 | 7.5 | 104 | 1652 | 2.7 | 102 | 671 | 1.1 | 97 | 1584 | 1.4 | 98 | 19 | LOQ-3000 |
| Corticosterone | 38 | 7.3 | 107 | 46.5 | 12 | 109 | 18.3 | 8.7 | 103 | 25.5 | 0.54 | 98 | 0.1 | LOQ-144 |
| 11-Deoxycotisol | 1.86 | 2.3 | 104 | 4.07 | 6.8 | 98 | 1.78 | 1.9 | 96 | 4.02 | 3.4 | 95 | 0.017 | LOQ-40 |
| Δ4-androstenedione | 2.13 | 0.74 | 102 | 4.72 | 4.2 | 97 | 3.39 | 6.8 | 94 | 5.98 | 3 | 93 | 0.042 | LOQ-1746 |
| Testosterone | 5.62 | 1.1 | 111 | 7.71 | 3.1 | 99 | 49.3 | 3 | 106 | 51.4 | 3 | 98 | 0.012 | LOQ-1732 |
| 17α-hydroxyprogesterone | 2.09 | 11 | 96 | 4.53 | 7 | 95 | 2.18 | 6.3 | 90 | 4.67 | 0.36 | 93 | 0.1 | LOQ-1513 |
| Progesterone | 3.48 | 1.3 | 100 | 7.99 | 4.9 | 98 | 3.89 | 5.5 | 92 | 8.33 | 5.2 | 94 | 0.036 | LOQ-500 |
LC-MS/MS limits of quantification (LOQ) and range of calibration curves were based on 10 standards prepared as for human serum analysis (20). (B) LC-MS/MS validation: Inter day control materials for this study were prepared in two pools of mouse serum (serum #72 and #73) spiked in low (Q Low) and high (Q High) levels. Results are the mean (n=3) of control materials analyzed in three batches.