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. 2021 Jan 11;14:544285. doi: 10.3389/fncel.2020.544285

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Copyright © 2021 Zhong, Yan, Li and Meng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HDAC9 negatively regulates miR-20a expression in vitro. (A) miR-20a expression in brain tissues of MCAO mice or MCAO mice treated with sh-HDAC9 detected by RT-qPCR (n = 8), normalized to U6. (B) miR-20a expression in OGD-treated neurons or OGD-treated neurons treated with sh-HDAC9 detected by RT-qPCR, normalized to U6. (C) HDAC9 enrichment in the miR-20a promoter region detected by ChIP. (D) Western blot analysis of HDAC9 protein in OGD-treated neurons transfected with sh-HDAC9, normalized to GAPDH. (E) miR-20a expression in OGD-treated neurons transfected with sh-HDAC9 detected by RT-qPCR, normalized to U6. *p < 0.05 vs. the sham, control, or sh-NC group. Measurement data (mean ± standard deviation) between the two groups were compared using an unpaired t-test. n = 3. ^#p < 0.05 vs. the sham + sh-HDAC9 group or the control + sh-HDAC9 group.