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Fluorescence microscopy images of epithelial cell layer in mono-culture Calu-3 and co-culture models after 4 (left) and 24 h (right) adhesion period. The tight junction ZO-1 proteins were stained in green and nuclei in blue. White squares are examples of cell fusion. Scale bars: 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
