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. 2021 Jan 20;13:617143. doi: 10.3389/fnmol.2020.617143

Figure 6.

Figure 6

Hypoxia promotes hippocampus-derived NSC proliferation and neuronal differentiation via the Wnt/β-catenin pathway. (A) Effects of hypoxia on NSC proliferation were examined by Nestin and BrdU staining. (B) Differentiation of NSCs was assessed by NeuN and GFAP. (C) Neuronal differentiation of NSCs was assessed by the expression of the immature neuron marker DCX. (D) The effects of hypoxia on Wnt3a expression in differentiated NSCs. The proportion of BrdU+ cells to Nestin+ cells (%) in proliferated NSCs (E) and the ratio of NeuN+ cells among total cells (%) in differentiated NSCs (F) were enhanced by hypoxia but not by hypoxia+ DKK-1. The ratio of GFAP+ cells among total cells (%) (G) was reduced by hypoxia. The ratio of DCX+ cells among total cells (%) (H) and the ratio of Wnt3a+ cells among total cells (%) (I) were enhanced by hypoxia but not by hypoxia + DKK-1. Western blots of Wnt/β-catenin pathway proteins expression in proliferated NSCs (J,K) and in differentiated NSCs (L,M). Western bolts were normalized to the control samples. All values are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA. n = 3 independent experiments. (A–D) Scale bar, 50 μm.