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. 2021 Jan 15;11:547436. doi: 10.3389/fphar.2020.547436

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Copyright © 2021 Cheng, Chen, Guo, Liu, Zhao, Lu, Yu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) EFEMP1 overexpressing (EFEMP1-OE) and (B) EFEMP1 knockdown HUVEC establishment and verification. High-content imaging visualization of HUVECs transfected with GFP-labelled GV492-EFEMP1/GV248-shEFEMP1 vectors was shown. qRT-PCR results of EFEMP1 relative mRNA levels showed a significant increase in GV492-EFEMP1-transfected HUVECs compared with that in GV492-CON335-transfected HUVECs and a significant decrease in GV248-shEFEMP1-transfected HUVECS compared with that in GV492-CON077-transfected HUVECs (**p < 0.01). EFEMP1 protein levels in the four HUVEC groups were tested using western blotting. EFEMP1 protein expression in GV492-EFEMP1-transfected HUVECs increased significantly compared with that in control CON335 and decreased in GV248-shEFEMP1-transfected HUVECS compared with that in control CON077 (**p < 0.01).