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. 2021 Jan 15;11:547436. doi: 10.3389/fphar.2020.547436

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Copyright © 2021 Cheng, Chen, Guo, Liu, Zhao, Lu, Yu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of EFEMP1 on HUVEC proliferation. CON335 HUVECs, EFEMP1-OE HUVECs, CON077 HUVECs, and shEFEMP1 HUVECs were stained using EdU and Hoechst. EdU-positive cells were highlighted in red and nuclei in blue, following Hoechst staining. Proliferation capacities were analyzed by calculating the percentage of EdU-positive cells. Three independent experiments were performed, and five fields were randomly selected for statistical analysis in each experiment. **p < 0.01.