Skip to main content
. 2021 Jan 11;11:593225. doi: 10.3389/fphar.2020.593225

FIGURE 4.

FIGURE 4

Ech protects H9c2 cells against H/R-induced injury. H9c2 cells were exposed to increasing doses (0, 1, 5, 10, 20, 40, and 80 μm) of Ech. (A) The cytotoxicity of Ech in H9c2 cells was evaluated using CCK-8 assay. H9c2 cells were exposed to H/R and then treated with Ech (10 and 20 μm), (n = 3). (B) CCK-8 assay results showing the protective effect of Ech on H/R-induced inhibition of H9c2 cell viability (n = 3). (C) Results showing the inhibitory effect of Ech on H/R-induced LDH release (n = 3). (D) Representative images and quantification of TUNEL staining showing the protective effect of Ech on H/R-induced H9c2 cell apoptosis (n = 3). (E) Analysis of caspase-3 activity showing the reduced activity of caspase-3 in H9c2 cells treated with H/R and Ech relative to cells treated with H/R only (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control (Ctrl) group; # p < 0.05 and ## p < 0.01 compared with the H/R group.