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. 2021 Jan 11;11:593225. doi: 10.3389/fphar.2020.593225

FIGURE 6.

FIGURE 6

Loss of YAP counteracts the protective effect of Ech on H/R-induced cell injury in vitro. (A, B) H9c2 cells were transfected with si-YAP and si-NC, and the silencing efficiency was determined by qRT-PCR and western blot analyses. After exposure to H/R, H9c2 cells were treated with Ech (10 μm) for 24 h (n = 3). (C) Results of CCK-8 assay showed that silencing of YAP abrogated the protective effect of Ech on H/R-induced cell viability inhibition (n = 3). (D) The inhibitory effect of Ech on H/R-induced LDH release was blocked by YAP knockdown (n = 3). (E) Representative images and quantification of TUNEL staining showing that the protective effect of Ech on H/R-induced H9c2 cell apoptosis was counteracted by si-YAP transfection (n = 3). (F) Analysis of caspase-3 activity showing the increased activity of caspase-3 in H9c2 cells treated with si-YAP, H/R, and Ech relative to H9c2 cells treated with H/R and Ech (n = 3). ***p < 0.001 compared with the si-NC and Ctrl groups; ## p < 0.01 compared with the H/R group; && p < 0.01 compared with the H/R + Ech + si-NC group.