Skip to main content
. 2021 Jan 13;14:589650. doi: 10.3389/fnins.2020.589650

FIGURE 4.

FIGURE 4

Effects of 24-h olanzapine treatment on protein expression of GFAP, S100B, TLR4 signaling, and ER stress and effect of 72-h olanzapine treatment on pyroptosis signaling in cultured human astrocytes. (A,C,E,G) Representative Western blot and densitometry analysis of GFAP, S100B, TLR4, MyD88, phospho-p65, MMP-9, phospho-p38, and phospho-ERK1/2 (44 and 42 kd); phospho-PERK, ATF6, IRE1, GRP78/BiP, and GSDMD (53 and 29 kd); CASPASE-1 (48 and 20 kd); and NLPR3 in cultured human astrocytes. (B,D,F,H) Western blot analysis of protein expression of GFAP, S100B, TLR4, MyD88, phospho-p65, MMP-9, phospho-p38, and phospho-ERK1/2 (44 kd); phospho-PERK, ATF6, IRE1, GRP78/BiP, and GSDMD (29 kd); CASPASE-1 (20 kd); and NLPR3. All data were presented as mean ± SEM. Statistical significance was defined as p < 0.05 (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. control). Con, control; OLZ, olanzapine.