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Protein Ki67 in HeLa cells are stained by (A) direct immunofluorescence (IF), (B) indirect IF, and (C) cleavable fluorescent tyramide (CFT). The images in (A–C) are captured with the exposure time of 1 s, 300, and 15 millisecond, respectively. (D) Normalized staining intensities of 30 different positions in (A–C). The y-axis in (D) is on a logarithmic scale. Scale bars, 25 μm.
