TABLE 2.

Substrate specificity of GspInv.

(C6H10O5)n	Substrate	Linkage	Specific activity (U mg–1)
N = 2	Sucrose	O-α-D-glucopyranosyl-(1-2)-β- D-fructopyranoside	3380 ± 26
	Trehalose	O-α-D- glucopyranosyl -(1-1)-α- D- glucopyranoside	0
	Cellobiose	O-β-D- glucopyranosyl -(1-4)- D- glucopyranoside	0
	Maltose	O-α-D- glucopyranosyl -(1-4)- D- glucopyranoside	0
	Isomaltose	O-α-D- glucopyranosyl -(1-6)- D- glucopyranoside	0
N = 3	Raffinose	O-α-D- galactopyranosyl -(1-6)-D- glucopyranosyl -(1-2)-β-D- fructopyranoside	208 ± 14
	Melizitose	O-α-D- glucopyranosyl -(1-6)-D- fructofuranosyl -(1-4)-D- glucopyranoside	0
N = 4	Stachyose	O-α-D-fructofuranosyl-(1-6)-D-galactopyranosyl-(1-6)- D-galactopyranosyl-(1-6) -D-glucopyranoside	128 ± 10
N > 20	Inulin	O-β-D-fructofuranosyl-(2-1)-[D-galactopyranosyl]n–D-glucopyranoside	0