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. 2021 Jan 22;11:602530. doi: 10.3389/fimmu.2020.602530

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Copyright © 2021 Yang, Lode, Berzaghi, Islam, Martinez-Zubiaurre and Hellevik

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of cancer-associated fibroblasts (CAFs) on natural killer (NK) cell proliferation. (A) Representative flow cytometry dot plots of the different experimental groups, showing percentage of carboxy-fluorescein succinimidyl ester (CSFE)-labeled NK cells on a side-scatter axis. (B) Bars representing mean (± SD) values for NK cell proliferation obtained from experiments with three different CAF donors. NK cells/NFs co-cultures were used as positive control, and the three experimental groups of NK cells/CAFs co-cultures were compared with the normal fibroblast (NF) group. Statistical P-values between NFs and CAFs co-cultures were determined using one-way ANOVA with Tukey correction for multiple comparisons. ** indicates P < 0.01.