Figure 6.

Effects of radiation on cancer-associated fibroblast (CAF)-derived immunomodulators. (A, B) Protein levels of secreted TGF-β and PGE2 in culture supernatants measured by ELISA. (C) Intracellular IDO protein expression in CAF cell lysates analyzed by ELISA. In (C), positive control is non-irradiated CAFs stimulated with IFN-γ and negative control non-irradiated CAFs cultured without stimulation. Data represents mean (± SD) values from three (A), five (B) or four (C) different CAF donors. P-values between NFs and CAFs in (A, B) were determined using one-way ANOVA with Tukey correction for multiple comparisons, whereas P-values between groups of monocultures and co-cultures in (C) were determined using two-way ANOVA with Dunnett correction for multiple comparisons. *P < 0.05, **P < 0.01 and ***P < 0.001.