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. 2021 Jan 22;11:602530. doi: 10.3389/fimmu.2020.602530

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Copyright © 2021 Yang, Lode, Berzaghi, Islam, Martinez-Zubiaurre and Hellevik

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of cancer-associated fibroblasts (CAFs) on natural killer (NK) cell surface receptor expression. Following co-culturing with fibroblasts, isolated NK cells were analyzed by flow cytometry for their expression of NK cell surface markers (A) NKG2D, (B) NKp46, (C) DNAM-1, (D) NKG2A, (E) KIR2DL1 and (F) KIR3DL1. Results are presented as median fluorescence intensity (MFI) and data represents mean (± SD) values from experiments with three different CAF donors. Statistical P-values between NK/NFs and NK/CAF-groups were determined using one-way ANOVA with Tukey correction for multiple comparisons * and ** indicates P < 0.05 and P < 0.01, respectively.