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. 2021 Jan 22;11:602530. doi: 10.3389/fimmu.2020.602530

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Copyright © 2021 Yang, Lode, Berzaghi, Islam, Martinez-Zubiaurre and Hellevik

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of radiation on cancer-associated fibroblast (CAF) surface expression of checkpoint molecules. Surface expression of checkpoint ligands PD-L1, CD155, CD112, and HLA-E (MHC-I) were analyzed by flow cytometry on control and irradiated CAFs (iCAFs). Results are expressed as fold non-irradiated CAF median fluorescence intensity (MFI). Bars represent mean (± SD) values from two different CAF donors. Statistical P-values between control CAFs and irradiated CAF-groups were determined using two-way ANOVA with Dunnett correction for multiple comparisons. **P < 0.01 and ***P < 0.001.