FIGURE 1.

Inhibition of platelet activation by saponin. The data from three volunteers are presented. (A) Western blotting demonstrating the expression levels of the inflammatory mediators, such as ICAM-1, VCAM-1 and/or the platelet activation marker, such as CD62p and CD63, and the protein gray value of each group was compared to the control. Washed platelets were pretreated with saponin (200 μg/ml, 400 μg/ml) or not for 1 h, then stimulated with sCD40L (10 μM) for 30 min, platelets lysates were resolved in 10% SDS-PAGE or 12% SDS-PAGE and assessed for target protein. (B) The results indicated the measurements of CD62p on the surface of washed platelets by flow cytometry. (C) ELISA assay detected washed platelets supernatant to indicate release levels of TXB2. CD40L was used as a positive control for TXB₂ release by platelets. Following saponin treatment (400 μg/ml) TXB2 expression was reduced compared with that noted following CD40L treatment (10 μM). #p < 0.05 vs. CD40L.