FIGURE 2.

Inhibition of the constitutive expression of the PI3K/Akt pathway by saponin causes decreased platelet activity. The results of three volunteers are shown. Western blotting indicated protein expression levels of CD40, as well as of the PI3K/Akt signaling molecules PI3K, Akt and P-Akt, and the protein gray value of each group was compared to the control. Washed platelets were pretreated with saponin (200 μg/ml, 400 μg/ml) or not for 1 h, then stimulated with sCD40L (10 μM) for 30 min, platelets lysates were resolved in 12% SDS-PAGE and assessed for target protein.