Skip to main content
. 2021 Jan 13;11:570603. doi: 10.3389/fphar.2020.570603

FIGURE 3.

FIGURE 3

Saponin reduces the protein expression levels of the MAPK pathway in platelets. The results of three volunteers are shown. Western blotting indicated protein expression levels of CD40, as well as of the p-p38 and p-JNK, and the protein gray value of each group was compared to the control. Washed platelets were pretreated with saponin (200 μg/ml, 400 μg/ml) or not for 1 h, then stimulated with sCD40L (10 μM) for 30 min, platelets lysates were resolved in 12% SDS-PAGE and assessed for target protein.