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. 2021 Jan 13;11:570603. doi: 10.3389/fphar.2020.570603

FIGURE 4.

FIGURE 4

Saponin downregulated the protein expression levels of the NF-κB pathway proteins and caused the inhibition of the CD40 signaling pathway via induction of TRAFs. Saponin downregulated the protein expression levels of the NF-κB pathway proteins and caused the inhibition of the CD40 signaling pathway via induction of TRAFs. The results from three volunteers are shown. (A,C) Western blotting indicated protein expression levels of the NF-κB signaling molecules NF-κB, p-NF-κB and IκB, as well as the E3 ligase TRAF 2, and the protein gray value of each group was compared to the control. Washed platelets were pretreated with saponin (200 μg/m, 400 μg/ml) or not for 1 h, then stimulated with sCD40L (10 μM) for 30 min, platelets lysates were resolved in 12% SDS-PAGE and assessed for target protein. (B) Platelets lysates were detected by NF-κB activity assay and bars represented different NF-κB activity. *p < 0.05 vs. control. #p < 0.05 vs. CD40L.