FIGURE 5.

Saponin-mediated inhibition of ubiquitination is associated with the Ubiquitin E3 ligase TRAF2. Washed platelets were pretreated with saponin (400 μg/ml) or not for 1 h, besides washed platelets were treated with MG132 (10 μM) for 30 min after pretreated with saponin (400 μg/ml) for 1 h in MG132 group, then stimulated with sCD40L (10 μM) for 30 min, platelets lysates were resolved in 12% SDS-PAGE and assessed for protein level of TRAF2, K48, K63 and Ub.