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. 2021 Feb 6;12:2041731420986796. doi: 10.1177/2041731420986796

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — A. HE staining of the peritoneal sheet. Black arrow: mesothelial cells. B. HE staining of the intact ileal seromuscular layer. M: muscle layer; S: serosa layer. C. Histopathological assessment of the neobladder constructed by both methods. Similar to what was observed in the MNB (a), HE staining revealed a thick layer of urothelium-like cells lying on the luminal surface of MN-Exp (d) (black arrow). Immunohistochemical analysis showed UPK3 and CK7 were highly expressed in the MNB (b and c) and MN-Exp (e and f) (black arrow), while no such changes were observed in the MN-Ctrl (h and i) and MNI (k and l). Compared with the MNI (j), the villi marked by the black arrow in the MN-Ctrl (g) were not atrophied 3 months after surgery. UPK3 (n) and CK7 (o) were negative on the NP (m). MNB, mucosa of the normal bladder; MNExp, mucosa of the neobladder in the experimental group; MN-Ctrl, mucosa of the neobladder in controls; MNI, mucosa of the normal ileum; NP, normal peritoneum; UPK3, uroplakin III; CK7, cytokeratin 7.