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. 2021 Feb;64(2):224–234. doi: 10.1165/rcmb.2020-0228OC

Figure 1.

Figure 1.

Large conductance potassium (BK) channels are expressed in human pulmonary microvascular endothelial cells (HULEC5a) and human primary alveolar epithelial cells (HPAEpiC). (A) Relative mRNA expression (ΔCt) of the pore-forming α-1 subunit KCNMA1 and the auxiliary KCNMB1, -2, -3, -4 β-subunits in HULEC5a (n = 6) and HPAEpiC (A549 and H441; n = 4–7). (B) On the left: a representative IP and Western blot experiment: pore-forming KCNMA1 protein expression in HULEC5a and HPAEpiC cells (n = 3). On the right: a representative Western blot experiment showing that the anti-KCNMA1 BK-α1 antibody detected the protein at the expected size (100–130 kD; right lane) in HEK293 cells overexpressing the KCNMA1 BK-α1-subunit. Arrows depict expected band size. (C) Fold change in KCNMA1 gene expression in LPS-treated (6 h, 2 μg/ml) HULEC5a and HPAEpiC cells (n = 4). FC = fold change; M = molecular weight marker.