Figure 2.

rhMG53 suppresses cytokine storm and mitigates pyroptosis in influenza virus–infected lung tissue. (A–C) ELISA quantification of IFN-β (A), IL-6 (B), and IL-1β (C) from lungs of influenza virus–infected mice treated with saline or rhMG53 and killed 7 days after infection. Each point represents an individual mouse and bars represent mean values. Error bars represent SD. P values determined by unpaired t test. (D) Representative Western blots of NLRP3 and GSDMD in lung tissues derived from uninfected mice (control), mice infected with influenza virus (Day 7) receiving saline, and mice infected with influenza virus (Day 7) receiving rhMG53 treatment. GAPDH blotting served as a loading control. Each lane is a sample from an individual mouse. GSDMD = gasdermin-D; GSDMD-FL = full length GSDMD; GSDMD-N = N-terminal fragment of GSDMD; rhMG53 = recombinant human mitsugumin 53.