Fig. 4.

ALKBH3 is involved in the biogenesis of 5′-tRF-GlyGCC. a, b The expression of 5′-tRF-GlyGCC (a) and mRNA of ALKBH3 (b) in CRC cell lines and human colon mucosal epithelial NCM460 cells were measured by qRT-PCR. c The protein expression of ALKBH3 in human CRC and NCM460 cells. d The Spearman correlation between the levels of 5′-tRF-GlyGCC and the mRNA expression of ALKBH3 in CRC and NCM460 cells. e Cells were transfected with the vector control or PPB/ALKBH3 for 48 h, and the expression of ALKBH3 and 5′-tRF-GlyGCC were measured by qRT-PCR. f Cells were transfected with the sh-NC control or sh-ALKBH3 for 48 h, and then the expression of ALKBH3 and 5′-tRF-GlyGCC were measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, ** p < 0.01 compared with control