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. Author manuscript; available in PMC: 2021 Oct 9.
Published in final edited form as: Free Radic Res. 2020 Oct 9;54(8-9):620–628. doi: 10.1080/10715762.2020.1821883

Figure 3. Effects of NADPH on resorufin formation in the AR/HRP assay.

Figure 3.

Reaction mixes in 140 μL contained 50 mM potassium phosphate buffer (pH 7.7), 200 mM KCl, 0.5 mM sodium azide, 0.1 mM DETAPAC and AR/HRP (10 μM and 0.5 units/ml, respectively). Reactions were run in the absence (closed symbols) and presence of 200 μM NADPH (open squares). H2O2 (1.0 μM in 10 μL) was added to the reactions as shown by the arrow (closed and open circles). Each data point represents the mean ± SD (n = 6). Error bars fell within the symbol of each data point.