Table 1.
The absolute rates of H2O2 generation by liver microsomal preparations.
| Liver Microsomes | Rates of H2O2 generation (nmol/min/mg of protein)1 |
|---|---|
| SD female rats, control | 2.62 ± 0.20 (2.93)1 |
| SD male rats, control | 3.38 ± 0.32 (4.18, 3.73) |
| SD male rats, phenobarbital induced | 7.49 ± 0.54 |
| SD male rats, isoniazide induced | 4.07 ± 0.31 (4.27, 3.51) |
| SD male rats, dexamethasone induced | 12.27 ± 1.29 (14.62, 7.62) |
| SD male rats, clofibrate induced | 8.20 ± 0.46 (8.0) |
| human liver microsomes | 2.17 ± 0.25 |
1
All determinations were performed within the linear range of H2O2 formation over time after the addition of NADPH. The background rate of AR oxidation in the absence of microsomal protein was 0.17 nmol of H2O2/min (n = 18), which is far below the LLQ (see Fig 1, lower panel). In these assays, 0.5–2 μg of liver microsomal proteins were used to analyze H2O2 reaction rates. Reactions were performed under conditions indicated in the legend to Fig. 6. Each value is the mean ± SD, n = 6–12. The values in brackets were found with different lots of liver microsomes from SD rats (mean, n = 2–4).