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. 2020 Jul 10;15(1):476–487. doi: 10.1515/biol-2020-0050

Figure 2.

Figure 2

Effects of circ_001569 knockdown on the progression of OS cells. U2OS and HOS cells were transfected with si-circ_001569#1/#2 or si-NC. (a) The expression of circ_001569 was detected by qRT-PCR to evaluate the transfection efficiency of si-circ_001569#1/#2. (b and c) MTT assay was performed to assess the proliferation ability of U2OS and HOS cells. (d and e) Transwell assay was performed to detect the migration and invasion of U2OS and HOS cells. (f–i) The levels of EMT-related proteins (N-cadherin, E-cadherin and Vimentin) in U2OS and HOS cells were detected by WB analysis. *P < 0.05.