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. 2020 Jul 10;15(1):476–487. doi: 10.1515/biol-2020-0050

Figure 4.

Figure 4

Effects of miR-185-5p inhibitor and circ_001569 knockdown on the progression of OS cells. U2OS and HOS cells were cotransfected with si-circ_001569 and anti-miR-185-5p or their negative controls (si-NC and anti-miR-NC). (a) The expression of miR-185-5p was detected by qRT-PCR to evaluate the transfection efficiency of si-circ_001569 and anti-miR-185-5p. (b and c) The proliferation of U2OS and HOS cells was detected by the MTT assay. (d and e) The migration and invasion of U2OS and HOS cells were measured by the Transwell assay. (f–i) The protein levels of N-cadherin, E-cadherin and Vimentin in U2OS and HOS cells were detected by WB analysis. *P < 0.05.