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HG conditions in HK-2 cells induced ROCK2 expression but suppressed miR-30c-5p expression. (a and b) Levels of miR-30c-5p and ROCK2 were detected by qRT-PCR in HG-induced HK-2 cells. (c) Western blot assay was conducted to determine mature ROCK2 expression in vitro. NG: normal glucose (5.6 mmol/L d-glucose); HG: high glucose (30 mmol/L d-glucose); HO: high osmotic pressure solution (5.6 mmol/L d-glucose + 24.4 mmol/L d-mannitol). *P < 0.05.
