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. 2021 Feb 9;21:86. doi: 10.1186/s12870-021-02862-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Cold tolerance assay of PbrbHLH195-silenced Pyrus betulaefolia plants. a-c Phenotype of 1-month-old PbrbHLH195-silenced plants before and after cold treatment for 8 days (a). Electrolyte leakage (EL) (b) and malondialdehyde (MDA) concentrations (c) after cold treatment. d-g Chlorophyll fluorescence imaging of silenced plants and WT plants(d), Fv/Fm ratios (e), Chl content (f) and phenotype (g) of WT and pTRV-PbrbHLH195 silencing plants (pTRV-1, pTRV-2 and pTRV-3) at the end of the chilling stress. h-i In situ accumulation of H₂O₂ of WT and silencing plants, as revealed by histochemical staining with 3, 3-diaminobenzidine (DAB) (h) after cold treatment. Quantitative measurement of H₂O₂ (i) levels after cold treatment. The expression of PbrbHLH195 was detected by RT-PCR (j) and qRT-PCR (k) at 8d after cold treatment