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. 2018 Sep 22;13:327–334. doi: 10.1515/biol-2018-0040

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© 2018 Xiaowu Chen et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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Luciferase reporter assay. (A) Plasmid construction. TK, human thymidine kinase promoter; fluc, firefly luciferase; rluc, renilla luciferase; lpCYP19a: long promoter of tilapia cyp19a; lmpCYP19a: long methylated promoter of tilapia cyp19a; spCYP19a: short promoter of tilapia cyp19a; smpCYP19a: short methylated of tilapia cyp19a; NP: no promoter. (B) Luciferase assay. Different groups of reporter plasmid of cyp19a promoter plus pRL-TK (internal control reporter) were co-transfected into HEK293 cells. At 24 h post-transfection, the cells were lysed for the luciferase assay. The asterisks indicates P<0.05 between two groups.