Fig. 1.
Expansion and activation of cynomolgus macaque (MCM) B cells for regulatory T cell (Treg) expansion. B cell expansion from fresh or cryopreserved peripheral blood mononuclear cells (PBMCs) or splenocytes. Protocol 1 includes 10 lines, protocol 2 includes 14 lines and protocol 3 includes 18 lines from nine animals. (a) Schema for the expansion and activation of MCM B cells. (b) B cell growth (mean and standard error of the mean (s.e.m.) from day 0 until the harvest for each of the B cell expansion protocols. (c) Average cell number at the end of the B cell culture for each protocol. Average and s.e.m. are detailed at the top of each bar. (d) Fold expansion for each B cell protocol. (e) Expression of B cell characteristic markers [major histocompatibility complex (MHC) II, CD80, CD20, CD40] and assessment of T cell contamination for each B cell expansion protocol at different time‐points (day 0, restimulation and harvest).