Fig. 3.

Forkhead box protein 3 (FoxP3) expression and suppressive capacity of CD4⁺CD8⁺ DP T cells. (a) Comparison between the percentage of CD3⁺CD4‐CD8⁺ SP and CD3⁺CD4⁺CD8⁺ double‐positive (DP) T cells present in the regulatory T cell (T_reg) cultures at the harvest time‐point (days 14, 21 or 28) (n = 57). Study of the (b) FoxP3, (c) Helios expression and (d) suppressive capacity [mean and standard error of the mean (s.e.m.)] of total CD3⁺ T cells (black), CD3⁺CD4⁺CD8⁻ SP T cells (blue) and CD3⁺CD4⁺CD8⁺ DP T cells (red) (n = 2). (e) Correlation between the percentage of T_reg‐specific demethylated region (TSDR) demethylation in total CD3⁺ cells and the percentage of CD8⁺ single‐positive (SP) and CD4⁺CD8⁺ (DP) T cells present in culture. Each color represents a different T_reg line (n = 15). (f) Assessment of the secretion of interferon (IFN)‐γ, interleukin (IL)‐13 and IL‐17A in peripheral blood mononuclear cells (PBMCs) and cultured sBc‐T_regs (n = 5).