Fig. 6.

Restimulation of sBc‐regulatory T cells (T_regs) after cryopreservation. Study of cryopreserved and re‐expanded CD40L‐sBc T_regs. Representation of two T_reg lines from two different animals recultured under three different conditions. (a) T_reg growth after thawing. (b) Fold expansion after 9 days of culture. (c) CD25⁺forkhead box protein 3 (FoxP3)⁺ expression in CD3⁺CD4⁺ cells. (d) Percentage of CD4⁺CD8⁺ T cells. (e) Suppressive capacity of the T_reg lines cultured under each condition at the thawing day and days 2, 3, 4, 7 and 9 after reculture.