Table 1.

MCM B cell expansion and activation protocols

	Protocol 1	Protocol 2	Protocol 3
Culture duration	10 days	14 days	14 days
Restimulation with CD40L‐expressing K562 cells	Day 7	Day 10	Day 7
Medium feeding	Day 2, 4	Day 2, 4, 7	Days 2, 4, 10
CyA dose	2 µg/ml (days 0), 1 µg/ml (days 2, 4)	2 µg/ml (days 0, 2, 4), 1 µg/ml (days 7, 10)	2 µg/ml (days 0, 2, 4), 1 µg/ml (days 7, 10)
IL‐4 dose	0.8 ng/ml (days 0), 0.4 ng/ml (days 2, 4, 7)	0.8 ng/ml (days 0), 0.4 ng/ml (days 2, 4, 7, 10)	0.8 ng/ml (day 0), 0.4 ng/ml (days 2, 4, 7, 10)
GCV dose	10 µg/ml (days 0), 5 µg/ml (days 2, 4, 7)	10 µg/ml (days 0), 5 µg/ml (days 2, 4, 7, 10)	10 µg/ml (day 0), 5 µg/ml (days 2, 4, 7, 10)

MCM = Mauritian cynomolgus macaque; IL = interleukin; GCV = ganciclovir; CyA = cyclosporin A.