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. 2021 Feb 10;547:23–28. doi: 10.1016/j.bbrc.2021.02.016

Fig. 1.

Fig. 1

EGCG inhibits 3CL-protease activity of HCoV-OC43 and HCoV-229E. (A) Amino acid sequence alignment of HCoV-229E 3CL-protease, HCoV-OC43 3CL-protease and SARS-CoV-2 3CL-protease. (B) Detection of HCoV-OC43 proteins in media. RD cells were infected with HCoV-OC43. Seven days after infection, media was collected and were probed with anti-HCoV-OC43 antibody. (C) HCoV-OC43 virus particles from infected cell media showed 3CL-protease activity in a FRET-based 3CL protease assay. (D) HCoV-229E virus particles showed 3CL-protease activity. (E) EGCG treatment inhibits 3CL-protease activity of HCoV-OC43 virus particles. (F) EGCG treatment inhibits 3CL-protease activity of HCoV-229E virus particles.