Table 5.

Global trend: Complementary studies.

Article	Year/Journal	Impact Analysis
Nakagawa, K., & Kishimoto, T. “Unlabeled image analysis-based cell viability assay with intracellular movement monitoring”[31]	2019/Biotechniques	“Unlabeled optical metabolic imaging of cultured living cells. This imaging technique is based on motion vector analysis with a block-matching algorithm to compare sequential time-lapse images. Motion vector analysis evaluates the movement of intracellular granules observed with a phase-contrast microscope. This assay can measure cellular viability at a single-cell level without requiring any reagents”. In this research, human osteosarcoma U2OS cells, human colon carcinoma Caco-2 cells and human hepatoma HepG2 cells were used.
Wu, H. et al. “Electrical impedance tomography for real-time and label-free cellular viability assays of 3D tumour spheroids”[32]	2018/Analyst	“In silico and in vitro cell viability inside large cell spheroids can be monitored in real time and label-free with electrical impedance tomography (EIT). The results show the potential of EIT for non-destructive real-time and label-free cellular assays in the miniature sensor, providing physiological information in the applications of the 3D drug screening and tissue engineering.” MCF-7 breast cancer cells were used, and the liquid overlay technique was adopted to form cells spheroids on the hydrogel surface. Cell suspension with 1×104 cells were seeded onto each microplate well.
Parrish, J et al. “A 96-well microplate bioreactor platform supporting individual dual perfusion and high-throughput assessment of simple or biofabricated 3D tissue models”[33]	2018/Lab on a Chip	“Platform to address the experimental and in vivo disparity in throughput and both system complexity (by supporting multiple in situ assessment methods) and tissue complexity (by adopting a construct-agnostic format). It describes the potential of a scalable dual perfusion bioreactor platform for parenchymal and barrier tissue constructs to support a broad range of multi-organ-in-a-chip applications”. In this research human umbilical cord-derived vascular endothelial cells (HUVEC), bone marrow-derived mesenchymal stromal cells (MSC), human ovarian cancer cells and human foreskin-derived fibroblast were used.