Table 1.

The Independent Variables and the Characterization Data of the Factorial Design-Derived NFX-Loaded PLGA NPs

NP Formulae	Independent Variables		Characterization Data									Desirability Indices
	Polymer Type	Drug:Polymer Ratio	PS (nm)	PDI	ZP (mV)	EE (%)	Q30min (%)	Q12h (%)	P30min (%)	P12h (%)	HL (%)
NP1	Ester-terminated PLGA	1:0.5	356.13±2.13	0.14±0.02	−22.52±0.23	37.80±1.13	12.12±0.92	63.21±2.10	20.21±1.5	59.41±2.11	82.60±0.27	0.23
NP2		1:1	392.02±1.33	0.17±0.07	−30.43±1.50	79.24±0.91	10.96±1.42	58.23±1.52	17.39±1.13	53.31±1.92	82.55±0.17	0.60
NP3		1:1.5	438.21±2.49	0.19±0.05	−25.61±0.41	68.52±1.31	8.17±2.27	52.01±2.71	14.13±1.62	45.19±2.33	82.66±0.33	0.43
NP4		1:2	485.05±1.83	0.24±0.05	−26.83±0.67	53.68±0.72	6.57±1.61	43.15±1.91	10.06±1.62	40.12±2.22	83.12±0.14	0.31
NP5	Acid-terminated PLGA	1:0.5	176.07±2.62	0.17±0.06	−26.51±1.60	59.63±1.43	19.31±0.78	78.23±1.73	25.02±0.73	70.12±1.81	82.08±0.33	0.18
NP6		1:1	190.51±1.23	0.18±0.09	−33.62±0.18	91.72±1.23	16.65±1.22	71.20±2.24	21.05±0.43	65.01±1.73	81.93±0.14	0.57
NP7		1:1.5	245.13±3.28	0.19±0.09	−27.67±0.41	78.54±0.81	13.26±2.32	65.32±1.82	18.17±1.42	56.54±1.71	82.48±0.34	0.53
NP8		1:2	256.12±2.94	0.20±0.08	−28.62±0.86	60.47±1.12	9.09±1.43	55.32±2.53	13.31±1.61	52.12±2.11	82.96±0.13	0.51

Notes: Data are represented as mean ± SD, n = 3.

Abbreviations: NFX, norfloxacin; PLGA, Poly(lactic-co-glycolic acid); NPs, nanoparticles; PS, particle size; PDI, polydispersity index; ZP, zeta potential; EE%, drug entrapment efficiency percentage; Q_30min, drug percentage released after 30 minute; Q_12h, drug percentage released after 12 hours; P_30min, drug percentage permeated after 30 minutes; P_12h, drug percentage permeated after 12 hours.