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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: ACS Synth Biol. 2019 Dec 17;9(1):1–9. doi: 10.1021/acssynbio.9b00277

Figure 4.

Figure 4.

BEACON platform enables dynamic spatial and temporal control of optogenetic systems. (A) Representative images of HeLa cells cotransfected with the iLID-mito:iRFP670-micro system and OMM-CeNLuc (n = 4 cells) compared to control cells cotransfected with Cyto-CeNLuc (n = 4 cells). (B,C) Representative images (B) and quantification (C) of biologic light-induced iRFP670-micro translocation to iLID-mito on mitochondria. Pulsatile addition of FZ enables repeated activation of the iLID-mito system, while washing out the FZ results in rapid inactivation of iLID-mito. A 680 W/m2 pulse of 474 nm blue light source was used as a positive control for Cyto-CeNLuc. All experiments were conducted using 10 μM FZ. Data shown are the average of 3–6 cells per condition expressed as mean ± SEM. All experiments were performed in transiently transfected HeLa cells as indicated in the SI Materials and Methods.