| Step | Temperature | Time |
| Initial denaturation | 95°C | 15 min |
| Denaturation | 95°C | 10 s |
| Annealing | 59°C (see materials) | 30 s |
| Extension | 72°C | 30 s |
| Final extension | 72°C | 10 min |
| Melting curve | Ramp down from 95°C to 60°C |
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