Fig. 3.

STAT3 is required for the upregulation of SNHG16

(A) The expression of STAT3 under pcDNA3.1/STAT3 transfection was assayed in VSMCs using qRT-PCR and western blotting. (B) The impacts of STAT3 elevation and suppression on SNHG16 were explored using qRT-PCR. (C) Potential binding sites of STAT3 within the promoter of SNHG16. (D) Luciferase activity of several reporters constructed from the SNHG16 promoter region in VSMCs cotransfected with STAT3-overexpressing or STAT3-downregulating plasmids. (E) ChIP was used to assay the enrichment of STAT3 on E1, E2, and E3 of the SNHG16 promoter region compared with IgG. **P < 0.01. n.s.: not significant.