Supplementary Fig. 1.

miR-106-5p is required for the regulation of SNHG16 on STAT3 and functions in VSMCs

(A) Luciferase activity of STAT3-WT and STAT3-Mut reporters in VSMCs under transfection of NC mimics, miR-106b-5p mimics, miR-106b-5p mimic+pcDNA3.1, miR-106b-5p mimics+pcDNA3.1/SNHG16, or miR-106b-5p mimics+pcDNA3.1/SNHG16 (Mut) (with the miR-106b-5p site mutated). (B, C) RT-qPCR and western blot results of the STAT3 level in VSMCs of the indicated group. (D, E) The proliferation of VSMCs under transfection of miR-106b-5p mimics versus NC mimics was evaluated using EdU and CCK-8 assays. (F) Quantification of TUNEL-positive VSMCs under miR-106b-5p overexpression versus control. (G) Western blot assay of cleaved caspase-3, total caspase-3, Bax, and Bcl-2 in VSMCs under miR-106b-5p overexpression versus control. **P < 0.01. n.s.: not significant.