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. 2021 Jan 11;162(4):bqab007. doi: 10.1210/endocr/bqab007

Figure 4.

Figure 4.

Hepatic Argonaute 2 (Ago2) deficiency enhances the expression of the peroxisome proliferator–activated receptor α (PPARα) pathway. A, Messenger RNA (mRNA) expression of Tfam-mitochondrial genes in livers of liver-specific Ago2-deficiency (L-Ago2) wild-type (WT) (n = 9) and L-Ago2 knockout (KO) (n = 9) mice at age 34 weeks. B, Western blot analysis of PPARα protein expression in the liver of L-Ago2 WT (n = 6) and L-Ago2 KO (n = 6) mice at age 34 weeks. C, miR-27a and miR-27b expression in livers of L-Ago2 WT (n = 9) and L-Ago2 KO (n = 9) mice at age 34 weeks. D, PPARα and its target mRNA expression in livers of L-Ago2 WT (n = 9) and L-Ago2 KO (n = 9) mice at age 34 weeks. E, Western blot analysis of PPARα protein expression in the liver of L-Ago2 WT-SHAM (n = 3), L-Ago2 WT-VSG (n = 3), L-Ago2 KO-SHAM (n = 3), and L-Ago2 KO-VSG (n = 3) mice 4 weeks post surgery. F, miR-27a and miR-27b expression in livers of L-Ago2 WT-SHAM (n = 6), L-Ago2 WT-VSG (n = 6), L-Ago2 KO-SHAM (n = 7), and L-Ago2 KO-VSG (n = 6) mice 4 weeks post surgery. G, PPARα and its target mRNA expression in livers of L-Ago2 WT-SHAM (n = 6), L-Ago2 WT-VSG (n = 6), L-Ago2 KO-SHAM (n = 7), and L-Ago2 KO-VSG (n = 6) mice 4 weeks post surgery. NS, nonspecific band. Data are shown as mean ± SEM of group size (n). Statistical analyses were performed by unpaired 2-tailed test for A to D, by multiple t test for E, or by 2-way analysis of variance followed by Tukey post hoc test for F and G. *P less than or equal to .05, * P less than or equal to .01, *** P less than or equal to .001.